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Columns and cartridges for gas, ion, HPLC, low-pressure LC, and flash chromatography; includes pre-packed and open columns, packing, guard columns and accessories, and other supplies for flash chromatography.
Ascentis™ Express Guard Columns provide physical (filtration) and chemical protection for costly analytical columns without compromising the very high performance of Ascentis™ Express columns.
Due to a mixed retention mechanism, selectivity differences for polar groups are more pronounced than on C8 and C18 columns. C1 columns require 20-30% less organic modifier to provide retention similar to C18 columns.
The Ascentis™ C8 is a highly reproducible column with excellent selectivity towards polar compounds. The phase excels in highly aqueous mobile phases as well as high organic mobile phases.
Available in a variety of stationary phases that function in normal and/or reverse phase. MilliporeSigma™ Hibar™ HPLC Columns, 250-4.6 come with customized packing (please specify packing material) and are 250mmL x 4.6mmI.D.
A typical mobile phase used with normal-phase silica columns consists of a hydrocarbon solvent such as hexane or heptane, mixed with a relatively small percentage of more polar solvent.
Ascentis™ Express 90 Å Biphenyl columns can be used for basic, acidic, or neutral compounds with alternate selectivity from C18 and is especially useful for separating peaks that elute early on C18 and other phenyl phases.